• 健常人のNK細胞が3週間以上増殖を続け、数100倍から数1000倍に増殖します。
  • 血液20~50mLから分離した末梢血単核球(PBMCs)からNK細胞を培養するキットです。
  • NK細胞を精製することなく、また、feeder cellを用いることなく、培養できます。
  • 研究用試薬です。


  • NK細胞初期培地、カクテル
  • NK細胞初期培養フラスコ
  • NK細胞継代培地

  仕様書 (PDF file)


  • 培養バッグ(小・大)
  • 培養バッグ連結管



Application Data


  • Nickolas Chelyapov, Toai T Nguyen, and Rafael Gonzalezb., Autologous NK cells propagated and activated ex vivo decrease senescence markers in human PBMCs. Biochem Biophys Rep. 32:101380, 2022
    PMID: 36386442
  • Jen-Tsun Lin, Yi-Ching Chuang, Mu-Kuan Chen, Yu-Sheng Lo, Chia-Chieh Lin, Hsin-Yu Ho, Yen-Tze Liu, Ming-Ju Hsieh., Shuterin Enhances the Cytotoxicity of the Natural Killer Leukemia Cell Line KHYG-1 by Increasing the Expression Levels of Granzyme B and IFN-γ through the MAPK and Ras/Raf Signaling Pathways. International Journal of Molecular Sciences. 23(21):12816, 2022
    PMID: 36361609
  • Chu Thi Thao, Bui Viet Anh, Nguyen Van Phong, Nguyen Trung Kien, Nguyen Dac Tu, Doan Trung Hiep, Nguyen Thanh Liem, Hoang Thi My Nhung., Immune Cells Expansion from Peripheral Blood of Some Vietnamese Cancer Patients. VNU Journal of Science: Natural Sciences and Technology. 37(3):2588-1140, 2021
  • Hui Menga, Yi Ding, Enjie Liu, Wencai Li, Liang Wang., ZG16 regulates PD-L1 expression and promotes local immunity in colon cancer. Translational Oncology. 14(2):101003, 2021
    PMID: 33360840
  • Ikumi Katano,Ryoji Ito,Kenji Kawai,and Takeshi Takahashi., Improved Detection of in vivo Human NK Cell-Mediated Antibody-Dependent Cellular Cytotoxicity Using a Novel NOG-FcγR-Deficient Human IL-15 Transgenic Mouse. Frontiers in Immunology. 11:532684, 2020
    PMID: 33117338
  • Nguyen Thanh Liem, Nguyen Van Phong, Nguyen Trung Kien, Bui Viet Anh, Truong Linh Huyen, Chu Thi Thao, Nguyen Dac Tu, Doan Trung Hiep, Do Thi Hoai Thu, and Hoang Thi My Nhung., Phase I Clinical Trial Using Autologous Ex Vivo Expanded NK Cells and Cytotoxic T Lymphocytes for Cancer Treatment in Vietnam. Int J Mol Sci. 20(13):3166, 2019
    PMID: 31261712
  • Chu Thi Thao, Do Thi Hoai Thu, Bui Viet Anh, Truong Linh Huyen, Nguyen Van Phong, Nguyen Thanh Liem, and Hoang Thi My Nhung., Efficient Expansion of Human Umbilical Cord Blood- Derived NK Cells Ex Vivo without Requiring Feeder Layers. Biomed J Sci & Tech Res. 8:6739-6744, 2018
    DOI: 10.26717/BJSTR.2018.08.001711
  • Hoang Thi My Nhung, Bui Viet Anh, Truong Linh Huyen, Doan Trung Hiep, Chu Thi Thao, Phung Nam Lam, Nguyen Thanh Liem., Ex vivo expansion of human peripheral blood natural killer cells and cytotoxic T lymphocytes from lung cancer patients. Oncol Lett. 15:5730-5738, 2018
    PMID: 29552207
  • 照沼裕、芦葉恵介、鄧学文、茂野淳平、高根翼、鮫島葉月、照沼篤, 活性化NK細胞の大量培養法の開発とその有効利用の可能性. BIO Clinica. 31:55-61, 2016 [Japanese].
  • 鄧学文、芦葉恵介、照沼篤、高根翼、鮫島葉月、贄田美江、照沼裕, 培養NK細胞の投与による体内NK細胞の活性化. Medical Science Digest. 41, 42-45, 2015.
  • Deng X, Terunuma H, Terunuma A, Takane T, Nieda M., Ex vivo-expanded natural killer cells kill cancer cells more effectively than ex vivo-expanded gd T cells or ab T cells. Int Immunopharmacol. 22:486-491, 2014
    PMID: 25131561
  • Terunuma H, Deng X, Nishino N, Watanabe K: NK cell-based autologous immune enhancement therapy (AIET) for cancer. Stem Cells Regenerative Med. 9:9-13, 2013
    PMID: 24693203
  • Deng X, Terunuma H, Nieda M, Xiao W, Nicol A., Synergistic cytotoxicity of ex vivo expanded natural killer cells in combination with monoclonal antibody drugs against cancer cells. Int Immunopharmacol. 14:593-605, 2012
    PMID: 23063974
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